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Bacteriostatic Water: literature-reported ranges

Bacteriostatic water is sterile water with 0.9% benzyl alcohol, which suppresses microbial growth and allows a reconstituted vial to be entered more than once. The relevant literature here is pharmaceutical compounding and stability practice rather than pharmacology.

Read this first

Every figure on this page is a range reported in published literature or in a publicly documented study protocol. It is a record of what researchers used, not a recommendation, a therapeutic protocol, or advice. Peptide Intel Hub is an independent publication, sells nothing, and describes these compounds as in-vitro research reagents only.

Compound at a glance

Class
Sterile water containing 0.9% benzyl alcohol as a preservative
Reported half-life
Not applicable — a diluent, not an active compound
Routes used in the literature
Diluent for reconstituting lyophilised research peptides
Also written as
0.9% benzyl alcohol water for injection

What the published studies reported

Each row below describes one body of work. The model and population are part of the figure — a rodent per-kilogram quantity and a human trial quantity are not comparable.

Preservative concentration

Reported quantity
0.9% benzyl alcohol (9 mg/mL)
Frequency
Fixed formulation
Study duration
Typically labelled for up to 28 days after first entry

The 28-day figure comes from compounding guidance for multi-dose preserved vials.

Typical reconstitution volumes in published preparation protocols

Reported quantity
1–5 mL per lyophilised vial
Frequency
Once, at reconstitution
Study duration
Refrigerated storage at 2–8 °C thereafter

Volume choice sets concentration; it has no effect on the total peptide present.

Reconstitution maths

Add the diluent slowly down the inner wall of the vial rather than directly onto the powder cake, then swirl. Do not shake — mechanical agitation is a documented cause of peptide aggregation.

Open the reconstitution calculator

How to read these figures

  • Benzyl alcohol is contraindicated in neonates and is not appropriate for every preparation.
  • Plain sterile water contains no preservative and is single-entry only.
  • Some peptides are incompatible with benzyl alcohol and are prepared in plain water or dilute acetic acid instead.

Sources and citations

These are the published records the figures on this page are drawn from. Each one describes what researchers administered, in which model or population, and over what period. Quantities reported in a study are specific to that study's design and are not transferable guidance.

  1. [1]
    Bacteriostatic Water for Injection

    United States Pharmacopeia · USP-NF Monograph · 2018 · USP-NF M88830

    Official USP monograph defining Bacteriostatic Water for Injection as sterile water containing one or more antimicrobial preservatives (commonly 0.9% benzyl alcohol) with specifications for identity, sterility, and use.

    View the record
  2. [2]
    Bacteriostatic Water Injection, Solution — Prescribing Label

    DailyMed / Hospira, Inc. · FDA DailyMed drug label · 2020 · NDC 0409-1093

    FDA-approved product labeling specifying benzyl alcohol 0.9% as the bacteriostatic preservative concentration in Bacteriostatic Water for Injection and its indicated use as a diluent.

    View the record

Search the databases yourself

Read the Bacteriostatic Water research brief

Other supplies compounds

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© 2026 Peptide Intel Hub · Marbella · Educational research reference · For in-vitro research use only
Independent publication — we sell nothing. Supplier disclosure

Peptide Intel Hub is an independent educational publication. We are not affiliated with, owned by, or the same company as Regena Peptides and Regena.app (regena-peptides.com / regena.app). We do not sell, supply, ship or take payment for any compound. Because readers regularly ask where compounds discussed in published studies can be sourced for laboratory work, we list Regena Peptides and Regena.app as suppliers we have verified and trust — every batch is released with a lot-matched third-party certificate of analysis (HPLC purity and mass-spectrometry identity). Outbound links are marked nofollow/sponsored and are provided to help readers, not to sell.