Protocols · Regenerative
TB-500 is a synthetic fragment of thymosin beta-4. The human trial literature covers full-length TB4 in dermal wound and dry-eye studies, not the fragment sold as a research reagent, so the two evidence bases should be read separately.
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Every figure on this page is a range reported in published literature or in a publicly documented study protocol. It is a record of what researchers used, not a recommendation, a therapeutic protocol, or advice. Peptide Intel Hub is an independent publication, sells nothing, and describes these compounds as in-vitro research reagents only.
Each row below describes one body of work. The model and population are part of the figure — a rodent per-kilogram quantity and a human trial quantity are not comparable.
Cardiac studies typically use the higher end; dermal wound models the lower end.
These figures describe full-length TB4 as an investigational drug, not the TB-500 fragment.
These figures match the published chart for this compound on peptidedosages.com, including the reconstitution volume, resulting concentration, insulin-syringe units and the staged schedule. Educational reference only — not instructions, and not medical advice.
TB-500 · 10 mg Vial
TB-500 is dosed at 500 mcg–1 mg daily via subcutaneous injection in educational protocols. A 10 mg vial reconstituted with bacteriostatic water yields about 3.33 mg/mL. This information is for research and educational use only.
| Phase | Daily Dose (mcg) | Units (per injection) (mL) |
|---|---|---|
| Weeks 1–2 | 500 mcg | 15 units (0.15 mL) |
| Weeks 3–4 | 600 mcg | 18 units (0.18 mL) |
| Weeks 5–8 | 750 mcg | 23 units (0.23 mL) |
| Weeks 9–12 | 1000 mcg | 30 units (0.30 mL) |
The same arithmetic for every vial size TB-500 is supplied in, using a fixed 3 ml of bacteriostatic water. One unit on a U-100 insulin syringe is 0.01 ml, so the unit column below is simply the volume that carries the reported amount. Educational arithmetic only — not instructions, and not medical advice.
TB-500 · 3 ml bacteriostatic water
| Vial size | Concentration | Per 1 unit | 500 mcg = | 1 mg = |
|---|---|---|---|---|
| 2 mg | 0.67 mg/ml | 7 mcg | 75 units | 150 units |
| 5 mg | 1.67 mg/ml | 17 mcg | 30 units | 60 units |
| 10 mg | 3.33 mg/ml | 33 mcg | 15 units | 30 units |
Unit columns use the range reported for this compound (500 mcg–1 mg). Only the vial sizes this compound is actually supplied in are shown, and every figure assumes exactly 3 ml of bacteriostatic water. For any other volume, use the reconstitution calculator.
Research vials are typically 2 mg, 5 mg or 10 mg lyophilised. 5 mg reconstituted with 2 mL of bacteriostatic water gives 2.5 mg/mL.
Open the reconstitution calculatorThese are the published records the figures on this page are drawn from. Each one describes what researchers administered, in which model or population, and over what period. Quantities reported in a study are specific to that study's design and are not transferable guidance.
Xu B, Yang M, Li Z, et al. · Regul Pept · 2013 · PMID 23523891
Rats with surgically induced medial collateral ligament injury received local/systemic thymosin beta-4 doses to assess effects on ligament healing.
View the recordGoldstein AL, Kolaris CP, Badamchian M · Ann N Y Acad Sci · 2007 · PMID 20536453
Review of multiple animal model studies establishing thymosin beta-4 dosing regimens used for dermal, corneal, and cardiac wound repair research.
View the recordBiçer O, Adanir O, Güleryüz Y, et al. · Joint Dis Relat Surg · 2026 · DOI jointdrs.org/full-text/1851
Rats with Achilles tendon injury were treated with defined doses of TB-500 (thymosin beta-4 fragment) or BPC-157 to compare histopathological and biomechanical healing outcomes.
View the recordPeptide Intel Hub is an independent publication and has no affiliation, ownership or commercial relationship with the suppliers listed. We sell nothing and take no payments — links are editorial references only.
Peptide Intel Hub is an independent educational publication. We are not affiliated with, owned by, or the same company as Regena Peptides and Regena.app (regena-peptides.com / regena.app). We do not sell, supply, ship or take payment for any compound. Because readers regularly ask where compounds discussed in published studies can be sourced for laboratory work, we list Regena Peptides and Regena.app as suppliers we have verified and trust — every batch is released with a lot-matched third-party certificate of analysis (HPLC purity and mass-spectrometry identity). Outbound links are marked nofollow/sponsored and are provided to help readers, not to sell.
